Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
List of solvents used for analysis of Mtb lipids by 2D TLC
| Components | Ratio | No. of runs | |
|---|---|---|---|
| Direction I | |||
| System | |||
| A | Pet ether/ethyl acetate | 98:2 | 3 |
| B | Pet ether/acetone | 92:8 | 3 |
| C | Chloroform/methanol | 96:4 | 1 |
| D | Chloroform/methanol/water | 100:14:0.8 | 1 |
| E | Chloroform/methanol/water | 60:30:6 | 1 |
| Direction II | |||
| System | |||
| A | Pet ether/acetone | 98:2 | 1 |
| B | Toluene/acetone | 95:5 | 1 |
| C | Toluene/acetone | 80:20 | 1 |
| D | Chloroform/acetone/methanol/water | 50:60:2.3:3 | 1 |
| E | Chloroform/acetic acid (glacial)/methanol/water | 40:25:3:6 | 1 |
