Composite double‐plotted actograms of 1‐3‐day‐old (A) wild type (w1118), (B) GMR‐GAL4, (C) GMR>CHMP2BIntron5 flies, and (D) a scatter plot of the free‐running locomotor period of the three genotypes show that GMR‐driven expression of CHMP2BIntron5 causes a significant reduction in the free‐running locomotor rhythm (wild type—23.82 ± 0.03 hours (n = 29); GMR‐GAL4—23.67 ± 0.06 hours (n = 25); GMR>CHMP2BIntron5—23.11 ± 0.07 hours (n = 37); error bars—standard error of measurement; n.s.—not significant; ***—P < 0.001; ANOVA, Bonferroni pairwise comparison). The timing of 12:12 LD and DD cycles is shown by alternating black and white rectangles on top of the actograms and by light gray shading on the actograms, respectively