Figure 3.

ChL response of isolated neutrophils in the presence of plasma and without plasma stimulated by SharL1 and SharL1^muc. Peak values for ChL are shown as white diamonds (SharL1) and black diamonds (SharL1^muc). Values for the same neutrophil sample are connected with the dotted lines (four experiments were performed with blood from three healthy donors). Medians for SharL1 and SharL1^muc are indicated with horizontal bars (A). Typical kinetics of the ChL response of the isolated neutrophils with (B) and without (C) plasma after stimulation with SharL1 (grey) and SharL1^muc (black). Arrows show the time point when the bacteria were added. *Significant difference between the ChL stimulated by SharL1 and SharL1^muc (P < 0.05, paired t‐test). Maximum values of ChL stimulated by 0.15 NaCl and 0.15 NaCl + mucin were 4.23 + 0.78 and 5.03 + 1.27, correspondingly (those values did not depend on plasma addition to isolated neutrophils).