Figure 2.

Calpain inhibitors eliminate most p53(ΔCp44) in HCMV‐infected cells. Cells were treated with calpain inhibitors [E64d (100 µM) or ZLLH (100 µM)] at 48 h PI and harvested at the indicated times post treatment (PT). Polypeptides (40 μg/lane) were resolved by SDS‐PAGE. Blots were probed with p53 antibody (DO‐1) and reprobed with actin antibody. These results are representative of at least two independent biological replicates, each one in two technical replicates. Note that the film exposure time for these DO‐1 immunoblots was shorter than those for DO‐1 immunoblots in Figure 1. M, mock‐infected; V, HCMV‐infected. Arrowhead: p53(ΔCp44). kDa sizes: the relevant molecular weight markers