Figure 3.

The proteasome inhibitor MG132 enhances the abundance of p53(ΔCp44). Cells were treated with or without MG132 (10 µM) at 48 hours PI and harvested at the indicated time post treatment (PT). Blots were probed with p53 antibody (DO‐1) and reprobed with actin antibody. These results are representative of at least two independent biological replicates, each one in two technical replicates. Note that the film exposure for these DO‐1 immunoblots was shorter than that for DO‐1 immunoblots in Figure 1. M, mock‐infected; V, HCMV‐infected. Arrowhead: p53(ΔCp44). kDa sizes: the relevant molecular weight markers