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. 2019 May 22;1(7):404–414. doi: 10.1096/fba.2018-00075

Figure 2.

Figure 2

Polysome profiling analysis from multiple myeloma cell lines. Cytosolic extracts from cells were loaded onto a 7%‐47% Sucrose gradient and fractionated with recording constant UV (254 nm) monitoring. 40S, 60S, 80S and polysomes represent small and large ribosomal subunits, monosomes and multiple ribosomes per mRNA. A, MM1.S cells overexpressing ACA11 or control empty vector (pLKO.1). B, ACA11 knockdown in ACA11‐overexpressing H929 cells