Table 1.
Endogenous and 2H‐labelled cytokinins (CK) scanned for by liquid chromatography‐positive electrospray ionization‐tandem mass spectrometry (HPLC‐(ESI+)‐MS/MS) in HeLa cell pellets and supernatants. Labelled internal standards (OlChemim Ltd.; Olomouc, Czech Republic) were used to identify and quantify CKs
| Endogenous CK Fractions | 2H‐labelled internal standard |
|---|---|
| Nucleotides (RP) | |
| trans‐zeatin riboside‐5′‐monophosphate (tZRP) | 2H6[9RMP]DZR |
| cis‐zeatin riboside‐5′‐monophosphate (cZRP) | |
| Dihydrozeatin riboside‐5′‐monophosphate (DZRP) | |
| N 6‐benzyladenosine‐5′monophosphate (BARP) | |
| N 6‐isopentyladenosine‐5′monophosphate (iPRP) | 2H6[9RMP]iP |
| Ribosides (R) | |
| trans‐zeatin riboside (tZR) | 2H5[9R]Z |
| cis‐zeatin riboside (cZR) | |
| Dihydrozeatin riboside (DZR) | 2H3[9R]DZ |
| N 6‐isopentyladenosine (iPR) | 2H6[9R]iP |
| N 6‐benzyladenosine (BAR) | 2H7[9R]BA |
| Free bases (FB) | |
| trans‐zeatin (tZ) | 2H3DZ |
| cis‐zeatin (cZ) | |
| Dihydrozeatin (DZ) | |
| N 6‐isopentyladenine (iP) | 2H6iP |
| N 6‐benzyladenine (BA) | 2H7BA |
| ortho‐topolin (oT) | 15N4 oT |
| Kinetin (KIN) | 15N4K |
| Glucosides (GLUC) | |
| trans‐Zeatin‐O‐glucoside (tZOG) | 2H5 tZOG |
| cis‐Zeatin‐O‐glucoside (cZOG) | |
| Dihydrozeatin‐O‐glucoside (DZOG) | 2H7DZOG |
| trans‐Zeatin‐O‐glucoside riboside (tZOGR) | 2H5 tZROG |
| cis‐Zeatin‐O‐glucoside riboside (cZOGR) | |
| Dihydrozeatin‐O‐glucoside riboside (DZOGR) | 2H7DZROG |
| trans‐Zeatin‐7‐glucoside (tZ7G) | 2H5 tZ7G |
| trans‐Zeatin‐9‐glucoside (tZ9G) | 2H5 tZ9G |
| cis‐Zeatin‐9‐glucoside (cZ9G) | |
| Dihydrozeatin‐9‐glucoside (DZ9G) | 2H3DZ9G |
| Methylthiols (2MeS) | |
| 2‐Methylthio‐trans‐zeatin (2MeSZ) | 2H5MeSZ |
| 2‐Methylthio‐trans‐zeatin riboside (2MeSZR) | 2H5MeSZR |
| 2‐Methylthio‐N 6‐isopentenyladenine (2MeSiP) | 2H6MeSiP |
| 2‐Methylthio‐N 6‐isopentenyladenosine (2MeSiPR) | 2H6MeSiPR |