Table 3.

Cytokinin (CK) forms and concentrations detected in the 10⁻⁶ mol/L BAPR treated medium blank, the 10⁻⁹ and 10⁻⁶ mol/L BAPR treated HeLa supernatants (nmol/L), and intracellularly in the corresponding treated cell pellets (pmol g/FW). Presented values are means ± SE (n = 3). A separate ANOVA was performed for each CK form for the supernatant samples with time as the dependent variable followed by Tukey's post hoc analysis

Treatment	Sampling time (h)	CK concentration (nmol/L, supernatant; pmol/106 cells, pellet)							cZRP	tZRP
		BA	iP	cZ	BAR	iPR	BARP	iPRP
Treated medium (10−6 mol/L BAPR, no cell) control	6	1.72 ± 0.39NS	n.d.	0.39 ± 0.01NS	518.16 ± 24.77NS	0.27 ± 0.03NS	0.12 ± 0.03NS	n.d.	n.d.	0.10 ± 0.03NS
	12	1.51 ± 0.25	n.d.	0.39 ± 0.04	474.45 ± 9.22	0.26 ± 0.01	0.14 ± 0.02	n.d.	n.d.	0.10 ± 0.06
	24	1.77 ± 0.24	n.d.	0.41 ± 0.06	479.84 ± 31.84	0.27 ± 0.01	0.14 ± 0.03	n.d.	n.d.	0.21 ± 0.09
	48	1.49 ± 0.20	n.d.	0.43 ± 0.04	449.26 ± 9.72	0.28 ± 0.04	0.16 ± 0.01	n.d.	n.d.	0.12 ± 0.06
	72	2.41 ± 0.19	n.d.	0.33 ± 0.02	496.21 ± 1.06	0.26 ± 0.002	0.11 ± 0.03	n.d.	n.d.	0.09 ± 0.07
10−9 mol/L BAR	6	1.47 ± 0.19b	0.14 ± 0.02b	0.58 ± 0.13NS	0.52 ± 0.09NS	0.13 ± 0.03NS	0.92 ± 0.82NS	n.d.	n.d.	1.09 ± 0.73ab
	12	1.57 ± 0.14ab	0.24 ± 0.05b	0.61 ± 0.22	0.25 ± 0.03	0.13 ± 0.03	0.69 ± 0.45	n.d.	n.d.	1.92 ± 1.52a
	24	1.71 ± 0.10ab	0.22 ± 0.01b	0.59 ± 0.12	0.34 ± 0.04	0.15 ± 0.02	0.30 ± 0.06	n.d.	n.d.	0.53 ± 0.15b
	48	1.64 ± 0.02ab	0.38 ± 0.004b	0.72 ± 0.18	0.25 ± 0.02	0.22 ± 0.03	0.24 ± 0.11	n.d.	n.d.	0.72 ± 0.21ab
	72	1.99 ± 0.25a	1.27 ± 0.03a	0.48 ± 0.04	0.18 ± 0.04	0.29 ± 0.01	0.08 ± 0.04	n.d.	n.d.	0.31 ± 0.16b
	Pellet (72)	0.006 ± 0.006	n.d.	n.d.	0.003 ± 0.0006	0.006 ± 0.0004	n.d.	0.20 ± 0.03	0.004 ± 0.0008	0.001 ± 0.0002
10−6 mol/L BAR	6	121.97 ± 10.38c	0.03 ± 0.02c	0.46 ± 0.08NS	527.61 ± 27.56a	0.26 ± 0.08NS	0.98 ± 0.82NS	n.d.	n.d.	0.45 ± 0.27NS
	12	270.93 ± 7.06b	0.11 ± 0.01bc	0.27 ± 0.14	406.27 ± 63.60b	0.15 ± 0.01	0.26 ± 0.15	n.d.	n.d.	0.37 ± 0.17
	24	577.00 ± 23.85a	0.20 ± 0.02bc	0.28 ± 0.16	43.57 ± 8.30c	0.04 ± 0.02	0.18 ± 0.11	n.d.	n.d.	0.55 ± 0.26
	48	520.29 ± 27.00a	0.31 ± 0.06b	0.13 ± 0.07	33.72 ± 6.90c	0.02 ± 0.02	0.22 ± 0.04	n.d.	n.d.	0.45 ± 0.07
	72	514.50 ± 44.18a	1.29 ± 0.24a	0.34 ± 0.10	23.65 ± 3.31c	0.06 ± 0.03	0.69 ± 0.25	n.d.	n.d.	0.89 ± 0.09
	Pellet (72)	0.03 ± 0.005	n.d.	n.d.	0.04 ± 0.005	0.005 ± 0.0004	n.d.	1.09 ± 0.77	0.004 ± 0.00003	0.00098 ± 0.0002

Values with the same letters are not significantly different (P < 0.05).

NS indicates lack of significant difference in CK concentrations between all time points.