Figure 2.

Antiviral effect of GSH‐C4 in aged mice. Fifteen‐month‐old mice were infected with a mouse‐adapted strain of influenza A/Puerto Rico/8/34 (H1N1; PR8) and treated with Placebo (I + Placebo) or GSH‐C4 (I + GSH‐C4) as described in the Materials and Methods section. The graphs represent the percentages of body weight (A) and body temperature (B) vs time 0 recorded in mice during treatment with GSH‐C4 (squares) or Placebo (circles). At day 8 pi, viral M1 RNA copies were measured in lungs (C) by quantitative Real Time (qRT‐PCR) as described in the Materials and Methods section. Results are the mean ± SD of five mice, each run in duplicate (n = 10). (A) and (B): Multiple t test followed by Holm‐Sidak method: **P = 0.003; ***P = 0.0004 vs I + Placebo; (C): Unpaired data were analyzed with the Student's t test with Welch correction: **P = 0.003