Figure 4.

Expression of NRF2 and PDI genes in lung homogenate of young and aged mice and of infected aged mice 15‐month‐old mice were infected with a mouse‐adapted strain of influenza A/Puerto Rico/8/34 (H1N1; PR8) and treated with Placebo (I + Placebo) or GSH‐C4 (I + GSH‐C4) as described in the Materials and Methods section. Lung homogenate was obtained at day 8 pi. The fold change respect to Young (A and C) or to Ctr (mock‐infected mice) (B and D) was calculated from three biological replicates using 2^−ΔΔCt method with normalization of the raw data to the reference gene GAPDH. Data analysis was performed using the web‐based RT2 Profiler PCR Array data analysis software (SABiosciences). The error bars represent 95% confidence intervals. Unpaired t test with Welch's correction *P < 0.05, **P = 0.0094