Table 1.
Key resources
| Reagent or resource | Source | Identifier |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal anti‐NPC1L1 | Novus Biologicals |
Cat# NB400‐128; RRID: AB_10000815 |
| Rabbit polyclonal anti‐EGFP | Life technologies | Cat# A11122; RRID:AB_221569 |
| Rabbit polyclonal anti‐α‐tubulin | Abcam |
Cat# ab15246; RRID: AB_301787 |
| Donkey anti‐rabbit IgG‐horseradish peroxidase (HRP)‐conjugate | GE Healthcare | Cat# NA934V; RRID:AB_772206 |
| Virus strains | ||
| EGFP‐expressing adenovirus | Toyoda et al14 | N/A |
| NPC1L1‐EGFP‐expressing adenovirus | This paper | N/A |
| Chemicals | ||
| Ezetimibe | Sequoia Research Products | Cat# SRP04000e; CAS: 163222‐33‐1 |
| IAXO101 | Innaxon | Cat# IAX‐600‐001‐M005; CAS: 1202388‐64‐4 |
| Clophosome®‐ Clodronate Liposomes (neutral) | FormuMax Scientific | Cat# F70101C‐N |
| Control Liposomes for Clophosome® (neutral) | FormuMax Scientific | Cat# F70101‐N |
| Critical commercial assays | ||
| Cholesterol E‐test Wako Kit | Wako | Cat# 439‐17501 |
| Triglyceride E‐test Wako Kit | Wako | Cat# 432‐40201 |
| RNeasy Micro Kit | Qiagen | Cat# 74004 |
| Deposited data | ||
| Gene microarray dataset | This paper | GEO: GSE110285 |
| Expression profiling by array: Human liver biopsy of different phases from control to NASH | Ahrens et al15 | GEO: GSE48452 |
| Experimental models: organisms/strains | ||
| Mouse: C57BL/6 J | Japan SLC | C57BL/6JJmsSlc |
| Mouse: B6;D2‐Tg(APOE‐NPC1L1)20Lqyu/J | The Jackson Laboratory | JAX: 008408 |
| Oligonucleotides | ||
| A full list of Primers | This paper | See Table 2 |
| Recombinant DNA | ||
| The complete NPC1L1 cDNA | Yamanashi et al7 | GenBank: AY437867 |
| Others | ||
| Control fat diet: consisted of crude sources of protein (soybean waste, whitefish meal, and yeast), fat (cereal germ and soybean oil), and carbohydrate (wheat flour and corn) | CLEA Japan | Cat# CE‐2: http://www.clea-japan.com/en/diets/diet_a/a_03.html (Detail of ingredients) |
| High‐fat diet | CLEA Japan | Cat# D15002 |
| ViraPowerTM Adenovial GatewayTM Expression Kit | Invitrogen | Cat# K493000 |
| Adeno‐XTM Rapid Titer Kit | Clontech | Cat# 632250 |