Figure 5. Histological analysis of diaphragm muscles incubated with Tyrode alone and bufotenine. (A, B) Tyrode alone, (C, D) 21 μg/mL of bufotenine and (E-G) 210 μg/mL of butotenine. Observe the normal muscle morphology in Tyrode and bufotenine at lower concentrations. However, myonecrosis can occur at 210 μg/mL of butotenine, as observed in (F) and (G). d: delta lesion, e: edematous fiber, h: hypercontracted fiber, g: ghost fiber. Magnification: (A, C, E) = 100x; (B, D, F, G) = 400x.