Figure 5.

Emerging sequencing and imaging technologies. An unprecedented view of 3D postmortem tissue and stem-cell-derived organoid models is emerging through breakthrough techniques. These include single-cell DNA sequencing (Lodato et al. 2015) and RNA sequencing (Ecker et al. 2017). Our visualization of gene expression will be increasingly spatially resolved with more widespread use of techniques that can visualize hundreds or thousands of messenger RNA (mRNA) transcripts in tissue, including fluorescent in situ sequencing (FISSEQ) (Lee 2017) and highly multiplexed error-robust FISH (MERFISH) (Moffitt et al. 2016). Visualization will be enhanced by tissue-expansion methods (Chen et al. 2015; Ku et al. 2016; Wang et al. 2018) and methods like SWITCH that combine delipidation of tissue with multiplex antibody analysis (Murray et al. 2015).