Figure 2.

Regulation of miR 221-3p and miR-155-5p in M1- and M2-macrophages by TLR ligands. Macrophages differentiated from CD14⁺ cells from (A) HD or (B) RA blood were stimulated with either 300 ng/ml Pam3 (TLR2), 10 μg/ml PolyIC (TLR3) or 100 ng/ml LPS (TLR4) for 24 h. Changes in miR expression levels were measured by qRT-PCR. Values were normalized to RNU48, miR-103a-3p, or miR-15b-5p and presented as 2^−ΔCT by boxplot with min/max whiskers. N = 3–6, *p < 0.05 compared to untreated cells (ctrl).