Figure 3.

Gene expression profile of M2-macrophages transfected with miR-221-3p mimic plus/minus LPS stimulation. (A) RNA-seq was performed of in vitro generated M2-macrophages transfected with control miR (ctrl) or miR-221-3p mimic (221) and stimulated with LPS for 8.5 h or left untreated (NS). The heatmap displays the centered and scaled gene expression levels of genes differentially expressed between miR-221-3p transfected and control cells in LPS-stimulated conditions. To better visualize the differences across transfected and control cells, the expression levels were corrected for donor-specific effects (see RNA-seq methods in the Supplementary File 1). (B) Volcano plot showing the differentially expressed genes between cells transfected with miR-221-3p mimic and control cells in LPS-stimulated cells. Significant genes are colored in red (up-regulated) and blue (down-regulated). For clarity, only genes with an absolute log2-fold change larger than 1 are annotated.