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. 2020 Jan 27;10:3087. doi: 10.3389/fimmu.2019.03087

Figure 4.

Figure 4

Effects of miR-221-3p mimics on cytokine and chemokine release in M1- and M2-macrophages. (A) M1- and M2-macrophages differentiated from CD14+ cells from HD blood were transfected with mimic of miR-221-3p (221 mimic) or a respective control miR (ctrl mimic) and stimulated with 100 ng/ml LPS or left untreated. Secreted cytokines and chemokines were measured after 8.5 and 24 h by ELISA and values are expressed as mean ± S.D. N = 6–10, *p < 0.05. (B) M2-macrophages differentiated from CD14+ cells from blood of RA patients were transfected with mimic of miR-221-3p (221 mimic) or a respective control miR (ctrl mimic) and stimulated with 100 ng/ml LPS or left untreated. Secreted cytokines and chemokines were measured after 8.5 and 24 h by ELISA and values are expressed as mean ± S.D. N = 6–11, *p < 0.05. (C) Anti-inflammatory activity of generated M2-macrophages from HD or RA patients was calculated after 8.5 and 24 h LPS-stimulation by the ratio of secreted cytokines IL-10 to IL-6 or chemokines CXCL13 to IL-8. Values are expressed as mean ± S.D. N = 6–11, *p < 0.05.