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. 2020 Jan 27;10:3087. doi: 10.3389/fimmu.2019.03087

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Copyright © 2020 Quero, Tiaden, Hanser, Roux, Laski, Hall and Kyburz.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of combined miR-155-5p/miR-221-3p mimics on pro- and anti-inflammatory cytokine secretion in TLR4-stimulated M2-macrophages. Cells were differentiated from CD14⁺ cells derived from HD blood. (A) M2-macrophages were transfected with mimics of miR-155-5p and miR-221-3p, either alone (155 or 221 mimic) or combined (155/221 mimic) and a control miR (ctrl mimic). M2-macrophages were then stimulated with 100 ng/ml LPS or left untreated. Secreted cytokines were measured after 24 h by ELISA and values are expressed as mean ± S.D. N = 15–18, *p < 0.05. (B) Anti-inflammatory activity of generated M2-macrophages transfected with the mentioned mimic combination setup was calculated after 24 h LPS-stimulation by the ratio of secreted cytokines IL-10 to IL-12 or IL-10 to IL-6. Values are expressed as mean ± S.D. N = 15–18, *p < 0.05.