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Isolation of SVA strains. (A) Representative images of cytopathic effects and immunofluorescence assay. PK-15 cells infected with SVA HeNNY-1/2018 strain at 18 h post-infection. Cells were stained with primary antibody of porcine SVA positive serum. (B) Representative plaque morphology in PK-15 cells infected by HeNNY-1/2018 strain at 72 h post-infection. (C) One-step growth curve of three SVA isolates on PK-15 cells.
