Skip to main content
. Author manuscript; available in PMC: 2020 Feb 3.
Published in final edited form as: Cell Rep. 2020 Jan 7;30(1):9–17.e3. doi: 10.1016/j.celrep.2019.12.008

Figure 3. Induction of Invasion Components in DRG Neurons Promotes Regeneration.

Figure 3.

(A–C) Z-projection time-lapse images of avulsed DRG in Tg(sox10:gal4); Tg(uas:lifeact-gfp) animals expressing uas:src-mcherry (A), uas:CA-src-mcherry (B), or uas:CA-src-mcherry and treated with Taxol (C). Graphs show Lifeact-GFP intensity tracings throughout regeneration. The green arrow denotes a stable invasive structure. Red arrows and brackets represent peaks of Lifeact-GFP.

(D) Actin concentrate duration in Src DRG (n = 6), CA-Src DRG (n = 6), and CA-Src+Taxol DRG (n = 5).

(E) Regeneration outcomes in DRG expressing sox10:Src, sox10:CA-Src, or sox10:CA-Src+Taxol or radial glial expressing gfap:CA-Src.

(F) Percentage of time points the regenerating growth cone navigated using each actin organization.

Tukey’s HSD was used in (D) and two-way ANOVA was used in (F). Scale bar, 10 μm.