| Antibodies |
| Zrf-1 (anti-GFAP, mouse monoclonal) |
ZIRC |
RRID: AB_10013806 |
| Phospho-p44/42 MAPK (anti-ERK1/2 Thr202/204,
rabbit monoclonal) |
Cell Signaling |
Cat #: 9101S; RRID: AB_331772 |
| Alexa Fluor 647 goat anti-mouse |
Thermo Fisher |
Cat #: A-21235; RRID: AB_2535806 |
| Alexa Fluor 647 goat anti-rabbit |
Thermo Fisher |
Cat #: A-20991; RRID: AB_2535814 |
| Bacterial and Virus Strains |
| Mix & Go Competent Cells |
Zymo Research |
Cat #: T3007 |
| Chemicals, Peptides, and
Recombinant Proteins |
| Paclitaxel |
Acros |
Cat #: 328420050; CAS:
33069–62–4 |
| GM6001 |
Santa Cruz Biotechnology |
Cat #: sc-203979; CAS:
142880–36–2 |
| SU6656 |
Santa Cruz Biotechnology |
Cat #: sc-203286; CAS:
330161–87–0 |
| LR Clonase II Plus |
Thermo Fisher |
Cat#: 12538120 |
| Experimental Models:
Organisms/Strains |
| AB |
ZIRC |
Wildtype zebrafish strain |
| Zebrafish: Tg(ngn1:gfp)
|
McGraw et al.,
2008
|
ZFIN: ZDB-ALT-090806–1 |
| Zebrafish:
Tg(sox10:gal4)
|
Hines et al.,
2015
|
ZFIN: ZDB-FISH-150901–5454 |
| Zebrafish:
Tg(uas:lifeact-gfp)
|
Helker et al.,
2013
|
ZFIN: ZDB-FISH-150901–1674 |
| Zebrafish:
Tg(gfap:nsfb-mcherry)
|
Johnson et
al., 2016
|
ZFIN: ZDB-ALT-160630–2 |
| Zebrafish:
Tg(uas:syn-gfp)
|
Heap et al.,
2013
|
ZFIN: ZBD-ALT-130702–2 |
| Recombinant DNA |
|
p5E-UAS
|
Kwan et al.,
2007
|
http://tol2kit.genetics.utah.edu/index.php/Main_Page
|
|
p5e-gfap
|
Don et al.,
2017
|
|
|
pME-Src
|
Seiler et al.,
2012
|
|
|
pME-CA-Src
|
Seiler et al.,
2012
|
|
|
p3E-mCherry-pA
|
Kwan et al.,
2007
|
http://tol2kit.genetics.utah.edu/index.php/Main_Page
|
|
pDestTol2CG2
|
Kwan et al.,
2007
|
http://tol2kit.genetics.utah.edu/index.php/Main_Page
|
|
psELN05 (pTol2-uas:CA-Src-mCherry,
cmcl2:gfp)
|
This paper |
N/A |
|
psELN06 (pTol2-uas:Src-mCherry,
cmcl2:gfp)
|
This paper |
N/A |
|
psELN10 (pTol2-gfap:CA-Src-mCherry,
cmcl2:gfp)
|
This paper |
N/A |
| Software and Algorithms |
| ImageJ |
NIH |
RRID: SCR_003070; https://imagej.nih.gov/ij/
|
| MTrackJ |
Image Science |
https://imagescience.org/meijering/software/mtrackj/
|
| Slidebook |
3i |
RRID: SCR_014300; https://www.intelligent-imaging.com/slidebook
|
| Prism |
GraphPad |
RRID: SCR_002798; https://www.graphpad.com/
|
