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. 2020 Jan 31;8:e8472. doi: 10.7717/peerj.8472

Table 1. Chronic unpredictable stress schedule for Experiment 1 and Experiment 2.

Seven stressors were randomized; fish in the stressed group were exposed to the cycle of stressors twice over 7 days. Subjects were exposed to stressors twice a day at random times during the light period between the times of 9 a.m. and 4 p.m.

Experiment Day Stressor Time of day(Experiment 1) Time of day(Experiment 2)
1 Tank changes (3 times) 11 a.m. 12 p.m.
Cooling (23 °C, 30 min) 1 p.m. 1 p.m.
2 Lowered water (15 min) 3 p.m. 2 p.m.
Net chase (8 min + 15 min rest + 8 min) 4 p.m. 3 p.m.
3 Crowding (250 ml beaker, 60 min) 10 a.m. 10 a.m.
Heating (33 °C, 30 min) 2 p.m. 4 p.m.
4 Social isolation (250 ml individual beakers, 45 min) 11 a.m. 9 a.m.
Tank changes (3 times) 12 p.m. 11 a.m.
5 Cooling (23 °C, 30 min) 3 p.m. 11 a.m.
Lowered water (15 min) 4 p.m. 4 p.m.
6 Net chase (8 min + 15 min rest + 8 min) 1 p.m. 11 a.m.
Crowding (250 ml beaker, 60 min) 2 p.m. 4 p.m.
7 Heating (33 °C, 30 min) 10 a.m. 10 a.m.
Social isolation (250 ml individual beakers, 45 min) 12 p.m. 1 p.m.