Table 1. Chronic unpredictable stress schedule for Experiment 1 and Experiment 2.
Seven stressors were randomized; fish in the stressed group were exposed to the cycle of stressors twice over 7 days. Subjects were exposed to stressors twice a day at random times during the light period between the times of 9 a.m. and 4 p.m.
| Experiment Day | Stressor | Time of day(Experiment 1) | Time of day(Experiment 2) |
|---|---|---|---|
| 1 | Tank changes (3 times) | 11 a.m. | 12 p.m. |
| Cooling (23 °C, 30 min) | 1 p.m. | 1 p.m. | |
| 2 | Lowered water (15 min) | 3 p.m. | 2 p.m. |
| Net chase (8 min + 15 min rest + 8 min) | 4 p.m. | 3 p.m. | |
| 3 | Crowding (250 ml beaker, 60 min) | 10 a.m. | 10 a.m. |
| Heating (33 °C, 30 min) | 2 p.m. | 4 p.m. | |
| 4 | Social isolation (250 ml individual beakers, 45 min) | 11 a.m. | 9 a.m. |
| Tank changes (3 times) | 12 p.m. | 11 a.m. | |
| 5 | Cooling (23 °C, 30 min) | 3 p.m. | 11 a.m. |
| Lowered water (15 min) | 4 p.m. | 4 p.m. | |
| 6 | Net chase (8 min + 15 min rest + 8 min) | 1 p.m. | 11 a.m. |
| Crowding (250 ml beaker, 60 min) | 2 p.m. | 4 p.m. | |
| 7 | Heating (33 °C, 30 min) | 10 a.m. | 10 a.m. |
| Social isolation (250 ml individual beakers, 45 min) | 12 p.m. | 1 p.m. |