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. 2019 Dec 16;39(3):e102500. doi: 10.15252/embj.2019102500

Figure EV1. Phenotypic characterization of the ΔrnjA strain.

Figure EV1

  1. Growth of Bacillus subtilis wt (LK1371) and ΔrnjA (LK1381) strains in LB medium at 37°C.
  2. Morphology of wt (LK1371) and ΔrnjA (LK1381) strains as captured by electron microscopy of exponential phase cells (OD600 0.5).
  3. Quantification of cells length of exponentially growing (OD600 0.5) wt (LK1371) and ΔrnjA (LK1381) cells stained with a membrane dye (NileRed, Sigma‐Aldrich). The analysis was performed on two sets consisting of 100 cells each. Due to technical considerations, the length of only non‐spiral cells was measured. The length of the cells was analyzed by Fiji ImageJ.
  4. Total RNA synthesis wt (LK1371) and ΔrnjA (LK1381) strains normalized to cell density (OD600). The data points are averages from two independent experiments (each done in technical duplicates); the error bars show the range.

Source data are available online for this figure.