Figure 3. Function of pMΦ‐ and fetal monocyte‐derived AM during homeostasis.

• A
  Representative flow cytometric analysis of BAL from Csf2ra ^−/− recipients 7 weeks after neonatal i.n. transfer of pMΦ or fetal Mo from CD45.1⁺ E14.5 fetal livers, 7‐week‐old un‐transferred Csf2ra ^−/−, WT (CD45.2) mice. Pre‐gated viable CD45⁺ singlets.
• B, C
  Numbers of donor‐derived AM in the BAL (left) and lung (right) of adult Csf2ra ^−/− recipients 7 weeks (B) or 1 year (C) after neonatal transfer of cells described in (A) (n = 7 mice). Age‐matched Csf2ra ^−/− (KO) (n = 5 mice) and WT (n = 4 mice) were included as negative and positive controls.
• D–G
  Total protein and total eFluor780⁺ events in the BAL of adult Csf2ra ^−/− recipients 7 weeks (D, E) or 1 year (F, G) after neonatal transfer of cells described in (A) (n = 7 mice). Age‐matched Csf2ra ^−/− (KO) (n = 5 mice) and WT (n = 4 mice) were included as negative and positive controls.

Data information: The data are representative of three independent experiments. In (B–G), data are presented as mean ± SEM, and ANOVA (one‐way) was used: ns, not significant; *P < 0.05, **P < 0.01, ***P < 0.001.