Figure 4. Function of pMΦ‐ and fetal monocyte‐derived AM during infection.

• A, B
  Body weight (A) and temperature (B) measurement of influenza virus (20 pfu of PR8 strain)‐infected Csf2ra ^−/− recipients 24 weeks after neonatal transfer of pMΦ or fetal Mo from E14.5 fetal livers (n = 7 mice from each transferred group), 24‐week‐old un‐transferred Csf2ra ^−/− (n = 5), and WT (n = 8).
• C
  Oxygen (O₂) saturation in influenza‐infected mice described in (A, B) 7 days post‐infection (p.i.) (n = 5 mice from Csf2ra ^−/− group; n = 7 mice from each transferred group; n = 8 from WT group).
• D
  Survival curve of influenza‐infected mice described in (A, B) (n = 5 mice from Csf2ra ^−/− group; n = 7 mice from each transferred group; n = 8 from WT group).
• E
  Total eFluor780⁺ events in the BAL of influenza‐infected mice described in (A, B) 10 days p.i. (n = 3 mice from each group).
• F
  Representative flow cytometric analysis of Csf2ra (CD116) expression on different fetal precursors from WT embryos. The solid gray histogram represents the fluorescence minus one (FMO) controls, the open black histogram represents stained samples. The gray number represents mean fluorescence intensity (MFI) of FMO controls, the black number represents MFI of stained samples. E10.5 YS cells were pooled from 8 to 10 embryos, E14.5 lung cells were pooled from 3 to 4 embryos, and E14.5 liver cells were from individual embryo.
• G
  Representative flow cytometric analysis of Csf2ra expressions on pMΦ‐ and fetal Mo‐derived AM in Csf2ra ^−/− recipients 4 weeks after transfer of pMΦ or fetal Mo from E14.5 fetal livers. AM from 4‐week‐old WT mice were included as controls.

Data information: In (A–E), the data are representative of three independent experiments. In (F, G), the data are representative of two independent experiments. In (A–E), data are presented as mean ± SEM, and ANOVA (one‐way) was used in (C, E): ns, not significant; **P < 0.01, ***P < 0.001.