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Metacodon analysis of 21‐nt RPFs in libraries prepared with CHX/ANS (blue) showing an increase in ribosome density at tripeptide motifs that undergo slow peptide bond formation (Schuller et al, 2017) compared to libraries prepared with CHX/TIG. P‐value was determined by Student's t‐test between CHX‐TIG and CHX‐ANS datasets on the tripeptide motifs indicated (*P = 0.0363, n = 3).
