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. 2019 Dec 20;39(3):e103365. doi: 10.15252/embj.2019103365

Figure EV6. Elongation of AAA is slower than AAG on MFK5 initiation complexes.

Figure EV6

  1. TLC showing peptide bond formation of MFK5 messages on inhibitory AAA codons (red) and control AAG codons (green). Samples were diluted 1–4 μl in water (top) or undiluted (bottom) to better resolve individual products on TLC. MFK5 AAG complexes are making longer lysine peptides (indicated by higher bands on the TLC) than AAA at early time points.
  2. Metacodon analysis of 21‐nt RPFs in libraries prepared with CHX/ANS (blue) and CHX‐TIG (black) showing an increase in ribosome density at polylysine motifs (KKK) comprised of both AAA and AAG codons. P‐value was determined by Student's t‐test between CHX‐TIG and CHX‐ANS datasets on the tripeptide motifs indicated (*P = 0.0204, n = 3).
  3. Metacodon analysis of 21‐nt RPFs in libraries prepared with CHX/ANS (blue) showing an increase in ribosome density at polylysine motifs (KKK) comprised of only AAG codons indicative of slow peptide transfer compared to libraries prepared with CHX‐TIG (black). P‐value was determined by Student's t‐test between CHX‐TIG and CHX‐ANS datasets on the tripeptide motifs indicated (*P = 0.0117, n = 3).