Skip to main content
. 2020 Jan 10;39(3):e102525. doi: 10.15252/embj.2019102525

Figure EV2. Adipocyte EV modify melanoma cell mitochondrial dynamics, a process that is dependent on FAO and promotes aggressiveness.

Figure EV2

  1. Human SKMEL28 and murine B16BL6 cells were exposed to 3T3‐F442A EV (3T3‐EV) and treated, or not, with Mdivi‐1. Cell migration was then evaluated in Boyden chamber assays. Bars and error bars represent means ± SEM (n = 6); statistically significant determined by one‐way ANOVA with post hoc Tukey's test, ***P < 0.001, ns: non‐significant.
  2. 1205Lu cells were exposed to adipocyte EV from lean (ND) or obese (HFD) mice for 48 with, or without, Etomoxir for the last 24 h. Cells were then stained with a MitoTracker probe, fixed, and observed by confocal microscopy. Arrows indicate the presence of mitochondria in membrane protrusions. Underneath each original image, an image depicting the outline of cells in dotted lines is shown. Scale bar: 20 μm.