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. Author manuscript; available in PMC: 2020 Feb 3.
Published in final edited form as: Sci Signal. 2019 Sep 3;12(597):eaax3332. doi: 10.1126/scisignal.aax3332

Fig. 2. Hematopoietic and nonhematopoietic cells differentially contribute to OVA-induced airway inflammation and AHR in the absence of DGKζ.

Fig. 2.

(A to C) FlexiVent analysis of airway resistance after methacholine treatment (A), cytospin analysis of total immune cells in BAL fluid (B), and ELISA for cytokine abundance in BAL fluid (C) from OVA-challenged WT and DGKζ KO BM chimeras. Data are means ± SEM of seven to nine mice per group from three independent experiments. (D to F) FlexiVent analysis of airway resistance after methacholine treatment (D), cytospin analysis of total immune cells in BAL fluid (E), and ELISA for cytokine abundance in BAL fluid (F) from OVA-challenged Vav-Cre DGKζfl/fl mice and Vav-Cre controls. Lung resistance values (D) are means ± SEM of seven mice per group from two independent experiments. BAL cell numbers (E) and cytokine abundance (F) are means ± SEM of 13 to 14 mice per group from four independent experiments. (G and H) Cytospin analysis of total immune cells (G) and ELISA for cytokine amounts (H) in BAL fluid from OVA-challenged CD4-Cre DGKζfl/fl mice and CD4-Cre controls. Data are means ± SEM of 12 to 17 mice per group from at least two independent experiments. (I) Flow cytometry analysis of the ratio of adoptively transferred cytokine-producing WT OT-II and DGKζ KO OT-II CD4+ T cells in the spleen of OVA-sensitized congenic WT hosts after ex vivo restimulation with PMA/ionomycin. Data are means ± SEM of 10 mice per group from two independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; NS, not significant by two-way ANOVA with Bonferroni’s posttest (A and D), two-sided unpaired Student’s t test (B and E to H), Mann-Whitney U test (C), or one-sided Student’s t test (I).