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. 2020 Jan 30;13:921–930. doi: 10.2147/OTT.S235336

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© 2020 Geng et al.

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miR-944 and miR-181d-5p are targets of SNHG6. (A-D) miR-944 (A) or miR-181d-5p (C) expression in 60 pairs of ANTs and NSCLC tissues detected by qRT-PCR; miR-944 (B) or miR-181d-5p (D) expression in five NSCLC cell lines and Normal human bronchial epithelial cell line BEAS-2B detected by qRT-PCR. (E) RIP assays were performed with Ago2 antibody or normal IgG, and SNHG6, miR-944 and miR-181d-5p expressions in the immunoprecipitates were detected by qRT-PCR. (F and G) The wild-type or mutated miR-944 (F) and miR-181d-5p (G) binding sites in SNHG6 sequence. (H and I) A549 cells were co-transfected with luciferase reporter and miR-944 (H) or miR-181d-5p (I) mimics, and then luciferase activity was detected after 48 h. Results were analyzed with one-way ANOVA followed by Student-Newman-Keuls test and shown as mean±SD; *p<0.05.

Abbreviation: NS, no significance.