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. 2020 Jan 30;16:327–338. doi: 10.2147/NDT.S238826

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© 2020 Dong et al.

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Protective effect of APLN against apoptosis of neurons. Hippocampal neurons of epileptic models were transfected with pBI-CMV3-APLN overexpression, short hairpin RNA negative control (shRNA-NC), or interference APLN shRNA plasmids. Forty-eight hours post-transfection, apoptosis was determined by flow cytometry. (A) Representative figure of flow cytometry; (B) Statistical results of these groups. Data are presented as mean ± SEM of three independent experiments. ns, non-significant; ****P < 0.0001.