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. 2020 Jan 30;16:327–338. doi: 10.2147/NDT.S238826

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© 2020 Dong et al.

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Relative expression of miR-182 and APLN in neurons. Neurons were transfected with miRNA negative control (miR-NC), miR-182 mimics, or miR-182 inhibitors. Twenty-four hours post-transfection, the expression of miR-182 was examined by qRT-PCR, and the expression of APLN was examined by qRT-PCR and Western blotting. (A) Relative expression of miR-182 determined by qRT-PCR. (B) Relative expression of APLN gene determined by qRT-PCR. (C, D) Western blotting analysis for APLN protein expression. **P < 0.01, ****P < 0.0001.