Table 5.
Values of EC 50 and Emax in the control and test groups: resveratrol + HA-1077 in concentrations of 20 μM/l and 100 μM/l.
| n 1 | EC50 (M/l) | E max (%)2 | p | |
|---|---|---|---|---|
| Control (Res−) | 8 | 4.53 ± 1.2 × 10−8 | 100.0 | — |
| HA-1077 (20 µM/l) (Res−) | 8 | 1.49 ± 0.34 × 10−7∗p < 0.0001a | 87.0 ± 3.0 | ∗ p < 0.001a |
| HA-1077 (20 µM/l) + resveratrol | 12 | 2.49 ± 0.81 × 10−7 | 76.4 ± 4.0 | p < 0.0001b |
| HA-1077 (100 µM/l) + resveratrol | 10 | 5.02 ± 0.73 × 10−7 | 70 ± 5.4 | nsc |
1The number of CRC used for the calculation; 2Emax calculated as a % of tissue maximal response to PHE; ap value calculated value relative to control (Res−); bp value calculated against HA-1077 (20 μM/l) (Res−); cp value calculated against HA-1077 (20 μM/l) (Res+). The calculated values of the antagonist concentrations corresponding to 50% of the maximum effect are summarized in the table above. In the presence of HA-1077, they were 1.49 ± 0.34 × 10–7 M/l, 2.49 ± 0.81 × 10–7 M/l, and 5.02 ± 0.73 × 10–7 M/l. Significant EC50 differences were observed between the control group (Res−) and HA-1077 (Res−) at a concentration of 20 μM/l-4.53 ± 1.2 × 10–8 M/l. 1.49 ± 0.34 × 10–7 M/l (p < 0.0001). All data are means ± SD.