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. 2020 Feb 3;15(2):e0228062. doi: 10.1371/journal.pone.0228062

Fig 3. Melatonin reduces migration and c-Myc expression independently of miR-210.

Fig 3

(A) MDA-MB-231 cells transiently transfected with anti-miR-210 have been treated with melatonin (1 mM) for 48 h and subjected to transwell migration assays. Histograms represent cell migration rate. Data represent the mean ± SEM of the transwell area covered by migrated cells, performed in triplicates plus or minus melatonin (**p < 0.001, p*** < 0.0001 treatment versus control) (B) MDA-MB-231 cells transfected as described above were treated with melatonin for 48 h and protein extracts were subjected to Western Blotting with the indicated antibodies. Protein expression was quantified by ImageJ program and calculated relative to controls, normalized with the endogenous HSP90 and expressed as percentages (%) (C) MDA-MB-231 cells transfected as described above were treated with melatonin for 48 h and miRNA levels measured by qRT–PCR analyses. Results are shown as fold changes (mean±s.e.m.) relative to control cells, normalized on U6 level. (* p < 0.05, **p < 0.001, ***p < 0.0001 treatment versus control). SEM = Standard Error of Mean.