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. 2020 Feb 3;15(2):e0228380. doi: 10.1371/journal.pone.0228380

Fig 2. Gating strategy of lymphocytes and T-cell subpopulations.

Fig 2

(a) Representative flow cytometric dot plots illustrating the gating strategy of lymphocytes and T cells (CD3+4+ and CD3+8+). (b) Representative T-cell differentiation pattern with respect to CD45RA and CD62L expression and the effect of fingolimod treatment in CD3+4+ (left column) and CD3+8+ cells (right column). The top panels indicate a typical distribution at baseline while the lower panels show the radical redistribution after one year of fingolimod treatment. Naïve cells were gated as positive for both CD45RA and the ligand for lymph node homing, CD62L; central memory (CM) as CD45RA-CD62L+; effector memory (EM) as CD45RA-CD62L- and terminally differentiated effector memory RA-positive cells (TEMRA) as CD45RA+CD62L-.