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. 2019 Dec 18;295(5):1202–1211. doi: 10.1074/jbc.RA119.011051

Figure 5.

Figure 5.

Competitive steroid binding assays. A, steroid binding assays preformed on diluted human serum, using [3H] DHT as a tracer, and unlabeled IPI, DVT, estradiol, and testosterone at increasing concentrations to establish displacement curves. The relative binding affinity of IPI, DVT, and estradiol compared with testosterone we found to be 74, 1.5, and 34%, respectively. B, competitive steroid-binding assays assess IPI, DVT, estradiol, and testosterone at increasing concentrations to establish displacement curves with the addition of 1 mm zinc chloride. The relative binding affinity of IPI, DVT, and estradiol compared with testosterone in the presence of zinc was found to be 3.7%, no binding, and 6.6%, respectively.