Figure 6.

Ligand-dependent interaction between SHBG and fibulin-2. A, GST:fibulin-2 (1081–1157) was immobilized on GSH beads and incubated overnight with diluted serum containing 20 nm human SHBG, in the presence and absence of 100 nm estradiol, DVT, or IPI. The beads were washed and then boiled in SDS loading buffer for analysis by Western blotting. B, this graph shows the relative amount of SHBG immunoreactivity (mean ± S.D. in red with individual data points as black squares) for each condition as measured by densitometry of three pulldowns shown in the above Western blotting. Significant differences determined using an unpaired t test from the stripped control, to which no ligand was added, are as indicated; *, p < 0.05; **, p < 0.005.