Figure 7.

CV1 SHBG-dependent androgen response assay luciferase data indicating that SHBG restricts the bioavailability of testosterone and nonsteroidal ligands are capable of increasing the bioavailability in a dose-dependent manner. DVT was found to slightly increase the bioavailability of testosterone (T), whereas IPI was significantly more effective. Data are from three experiments expressed as mean ± S.D. in red with individual data points as black squares. An unpaired t test was applied to compare the statistically significant difference between experimental samples to the T + SHBG baseline. *, p < 0.05; **, p < 0.005;***, p < 0.0005. Heat treatment of SHBG confirmed that no androgens remained bound to SHBG after stripping and purification.