Figure 6.

H3K36me3 enrichment in gene bodies correlates with gene expression levels. A, C, and E, genome-wide accumulation of H3K36me3 around TSS and gene body. Heatmap analysis of H3K36me3 ChIP-seq signal in cells treated with vehicle (UNTR) or proteasome inhibitor for the region flanking ±5 kb relative to TSS (black arrow) and TTS (red triangle). A, all expressed genes; C, MG4H DEGs; E, MG24H DEGs. B, D, and F, metagene plot of H3K36me3 occupancy shows increased and decreased enrichment at up- and down-regulated genes, respectively. B, all expressed genes; D, MG4H DEGs; and F, MG24H DEGs. G, H, and I, expanded metagene plots TTS region ±5 show distinct differences in H3K36me3 occupancy between up- and down-regulated genes.