Figure 4. Effect of CPE on interaction of CLDN4 and YAP1 in OSCC cells.

(A) Effect of CPE (10 μg/ml) on protein interaction among CLDN4, YAP1 and ZO-2 was examined by immunoprecipitation. (B) Nuclear translocation of CLDN4, YAP1 and ZO-2 in CPE-treated OSCC cells with or without 4D3 treatment. (C) CLDN4 nuclear translocation in the ¹³¹I-surface labeled OSCC cells. Nuclear fraction was immunoprecipitated with 4D3 for detection with 4D3 and autoradiography (AR). (D) In vitro phosphorylation assay using recombinant human YAP1, ZO-2, CLDN4 and LATS1. Reaction solution was subjected to immunoblotting. Whole, whole cell lysate; Nuclear, nuclear fraction; CB, Coomassie blue; lamin, loading control for nuclear protein; GAPDH, loading control for whole cell lysate.