Table 3. Sensitivity of GBM models to p53-MDM2 inhibitors (EC₅₀ in μM).

Compounds	Pifithrin-μ	RG7112	YH239-EE	Nutlin 3A	MDM2 predicted copy number	p53 mutation status
HF2885	0.8531	0.04785	2.234	0.189	7.76	wt
HF2381	0.857	0.009132	1.038	0.2116	55.63	wt
HF2476	1.466	0.01356	0.4542	0.313	normal	wt
HF2941	1.361	0.04077	1.12	0.5516	normal	wt
HF3026	0.6887	0.1334	2.153	1.284	normal	wt
HF2998	0.8735	0.2632	2.8	1.809	normal	wt
HF2790	1.225	0.9405	3.879	6.943	normal	C242F
HF3177	1.024	0.8706	3.961	6.968	normal	M133T
HF2906	0.7136	0.6126	4.61	7.93	normal	R175H
HF2876	3.784	0.0818	1.959	10.73	normal	wt
HF2303	3.353	0.9435	3.881	17.02	normal	G245S
HF3013	3.343	1.007	6.758	100	normal	V272M

The sensitivity of 12 different patient-derived GBMs in 3D neurosphere culture was determined by dose-response testing with each compound in triplicate. Mean EC₅₀ values in micromolar are indicated in Table 3.