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. 2020 Jan 28;11(4):362–377. doi: 10.18632/oncotarget.27406

Figure 5. PMEPA1-d isoform inhibited TGF-β signaling in PC3 cells.

Figure 5

(A) PC3 cells were transfected with expression plasmids of PMEPA1 isoform c, d and e as indicated. And the transfected cells were harvested 72 hours post transfection. The transcript levels of TGF-β responsive genes COL1A1, NEDD9 and THBS1 were detected with Q-PCR assay. (B) PC3 cells were transfected with specific siRNA against PMEPA1 isoform c, d and e. Q-PCR was used to detect the transcript levels of COL1A1, NEDD9 and THBS1. (C) The SMAD luciferase reporter assay was applied to assess the effects of PMEPA1 isoforms (c, d and e) on TGF-β signaling in PC3 cells.