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. Author manuscript; available in PMC: 2020 Feb 3.
Published in final edited form as: J Vis Exp. 2019 May 10;(147):10.3791/59113. doi: 10.3791/59113

Table of Materials

Name of Material/ Equipment Company Catalog Number Comments/Description
Adhesive tape Leica #39475214
Alexa fluor 488-goat anti-Rabbit secondary antibody Invitrogen A-11034
Antifade Mountant with DAPI Invitrogen P36931
bovine serum albumin Sigma A2153
Coverslips Fisher Brand 12–545-E
Cryostat Leica CM1850
EDTA Sigma E6758
Fluorescence microscope Olympus BX51
Gelatin Sigma G1890
In Situ Cell Death Detection Kit Millipore S7165
Microscope slides Fisher Brand 12-550-15
OCT Compound Fisher Healthcare 23-730-571
Paraformaldehyde (PFA) Sigma P6148 
Phosphate buffered saline (PBS) Sigma P4417
Polyethylene glycol tert-octylphenyl ether Sigma T9284 Triton™ X-100
Proteinase K Invitrogen AM2542
Rabbit anti-Ki67 antibody Cell Signaling Technology 9129 Lot#:3; RRID:AB_2687446
Rabbit anti-pSmad1/5/9 antibody Cell Signaling Technology 13820 Lot#:3; RRID:AB_2493181
Sodium citrate Sigma 1613859
Sucrose Sigma S9378
Tris Sigma 10708976001