Adhesive tape |
Leica |
#39475214 |
|
Alexa fluor 488-goat anti-Rabbit secondary antibody |
Invitrogen |
A-11034 |
|
Antifade Mountant with DAPI |
Invitrogen |
P36931 |
|
bovine serum albumin |
Sigma |
A2153 |
|
Coverslips |
Fisher Brand |
12–545-E |
|
Cryostat |
Leica |
CM1850 |
|
EDTA |
Sigma |
E6758 |
|
Fluorescence microscope |
Olympus |
BX51 |
|
Gelatin |
Sigma |
G1890 |
|
In Situ Cell Death Detection Kit |
Millipore |
S7165 |
|
Microscope slides |
Fisher Brand |
12-550-15 |
|
OCT Compound |
Fisher Healthcare |
23-730-571 |
|
Paraformaldehyde (PFA) |
Sigma |
P6148 |
|
Phosphate buffered saline (PBS) |
Sigma |
P4417 |
|
Polyethylene glycol tert-octylphenyl ether |
Sigma |
T9284 |
Triton™ X-100 |
Proteinase K |
Invitrogen |
AM2542 |
|
Rabbit anti-Ki67 antibody |
Cell Signaling Technology |
9129 |
Lot#:3; RRID:AB_2687446 |
Rabbit anti-pSmad1/5/9 antibody |
Cell Signaling Technology |
13820 |
Lot#:3; RRID:AB_2493181 |
Sodium citrate |
Sigma |
1613859 |
|
Sucrose |
Sigma |
S9378 |
|
Tris |
Sigma |
10708976001 |
|