Figure 2.

Maf1 is regulated by PP2A/PP4 phosphatases and the TORC1 kinase. (a, b) Cells of the indicated genotypes were engineered to express Maf1–5FLAG and grown at 30°C in YES liquid medium supplemented with 0.1%, 1%, or 5% glucose. Protein extracts were prepared, and Western blotting of Maf1‐5FLAG was performed. Tubulin was used as a loading control. Representative images of repeat experiments are shown. (c) WT, torc1, and maf1Δ cells were cultured in YES medium with different percentages of glucose (0.1%, 1%, and 5%) at 25°C for the indicated days. Fivefold serial dilutions of the cells were then plated on YES agar medium containing 3% glucose for lifespan assays. (d) Lifespan assay was performed with cells of the indicated genotypes. Cells were cultured in YES liquid medium supplemented with 0.1% glucose at 30°C for the indicated days before plating on YES agar medium as described above. Representative images of repeat experiments are shown