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. 2019 Dec 18;19(2):e13085. doi: 10.1111/acel.13085

Figure 3.

Figure 3

Low insulin signaling during quiescence ameliorates proliferative potential and attenuates L1 aging. (a) Recovery and developmental time for the wild‐type strain and the daf‐16(mu86) mutant after one and four days of arrest. Average values per experiment are indicated with a black dot, and values from single animals are indicated with a colored dot. We performed t test on the averages of 3 biological replicates (* p < .05). (b) Recovery and developmental timing for the wild‐type strain and the daf‐2(e1370) mutant after 8 and 14 days of arrest. We performed t test on the averages of 3 biological replicates (* p < .05, *** p < .001). (c) Recovery and developmental timing for the wild‐type strain, the daf‐16(mu86) mutant and the double‐mutant daf‐2(e1370);daf‐16(mu86) mutant after 1 and 4 days of arrest. (d) Seam cell division in wild‐type and daf‐16 larvae during recovery after one or four days of arrest. Values represent the percentage of animals showing division in three biological replicates. (e) Recovery and developmental timing for animals categorized as having low or high DHE staining after 8 days of arrest. We performed one‐way ANOVA on the averages of 4 biological replicates (** p < .01). (f) Quantification of ROS accumulation in the wild‐type strain, daf‐2 (e1370), and daf‐16(mu86). Plots show mean (±SD) of 3–4 biological replicates. For days 1, 4, and 8, we performed one‐way ANOVA on the averages of biological replicates, followed by Dunnett´s Multiple Comparison test to detect significant differences between the mutants and the wild‐type. For day 14, we performed t test (** p < .01). (g) Quantification of amyloids in the wild‐type strain, daf‐2(e1370), and daf‐16(mu86). Plots show mean (±SD) of four biological replicates. Statistics were performed as in (g) (* p < .05 and *** p < .001). (h) Recovery and development for larvae arrested during eight days in the presence of ammonium chloride and acridine orange (AO). (i) Recovery and development of wild‐type and daf‐16 mutant L1 arrested during four days in the presence of (AO)