Figure 4.

RIP3 is required for p62 accumulation‐enhanced myocardial necroptosis. (a) WT and RIP3 KO mice were subjected to 30 min cardiac ischemia and 4 hr reperfusion; then, the p62‐RIP1‐RIP3 complex and the RIP1‐RIP3 complex were examined by co‐immunoprecipitation. (b and c), Myocardial p62, p‐RIP3/RIP3, and p‐MLKL/MLKL were detected and quantitative by immunoblotting. (d and e) Myocardial necroptosis was evaluated by EBD uptake (red). (f) Serum LDH concentration in each group. (g and h) Representative Western blot and averaged data for cardiac or plasma HMGB1 levels in WT or RIP3 KO mice subjected to I/R injury. Scale bar = 20 μm. The values are the means ± SEM, n = 6 per group, *p < .05 versus the indicated groups