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. 2019 Dec 5;11(2):286–294. doi: 10.1111/1759-7714.13254

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© 2019 The Authors. Thoracic Cancer published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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The expression of OPN3 affects the migration and invasion of LUAD cells. (a and b) The basic endogenous expression of OPN3, including RNA and protein levels, in normal lung epithelial cell line (BEAS‐2B) and five lung adenocarcinoma cell lines (A549, HCC827, NCI‐H522, NCI‐H23, NCI‐H1975). (c and d) The expression of OPN3 in A549 cell lines was decreased by transfecting OPN3 siRNA (siOPN3 1#,2#,3#), and the expression of OPN3 in HCC827 cell lines was increased by transfecting OPN3 overexpressed plasmid, which was verified by western blot. (e) Wound healing experiments were performed in the A549 cells transfected with negative control siRNA (SiNC) and siOPN3 2# for 24 hours. (f) Transwell migration and invasion assays were performed in the A549 cells transfected with negative control siRNA (SiNC) and siOPN3 2#, the counts of cell passing through the chamber between the two groups were statistically different () SiNC, and () SiOPN3 2#. (g) Wound healing experiments were performed in the HCC827 cells transfected with pcDNA3.1(vector) and pcDNA3.1‐OPN3 for 24 hours. (h) Transwell migration and invasion assays were performed in the HCC827 cells transfected with pcDNA3.1(vector) and pcDNA3.1‐OPN3, the counts of cell passing through the chamber between the two groups was statistically different () vector, and () OPN3. *P < 0.05.

Inline graphic — The expression of OPN3 affects the migration and invasion of LUAD cells. (a and b) The basic endogenous expression of OPN3, including RNA and protein levels, in normal lung epithelial cell line (BEAS‐2B) and five lung adenocarcinoma cell lines (A549, HCC827, NCI‐H522, NCI‐H23, NCI‐H1975). (c and d) The expression of OPN3 in A549 cell lines was decreased by transfecting OPN3 siRNA (siOPN3 1#,2#,3#), and the expression of OPN3 in HCC827 cell lines was increased by transfecting OPN3 overexpressed plasmid, which was verified by western blot. (e) Wound healing experiments were performed in the A549 cells transfected with negative control siRNA (SiNC) and siOPN3 2# for 24 hours. (f) Transwell migration and invasion assays were performed in the A549 cells transfected with negative control siRNA (SiNC) and siOPN3 2#, the counts of cell passing through the chamber between the two groups were statistically different () SiNC, and () SiOPN3 2#. (g) Wound healing experiments were performed in the HCC827 cells transfected with pcDNA3.1(vector) and pcDNA3.1‐OPN3 for 24 hours. (h) Transwell migration and invasion assays were performed in the HCC827 cells transfected with pcDNA3.1(vector) and pcDNA3.1‐OPN3, the counts of cell passing through the chamber between the two groups was statistically different () vector, and () OPN3. *P < 0.05.