Figure 3.

ALCAM was the target of miR‐148b. (a) Prediction binding sites of ALCAM containing with miR‐148b. (b,c) ALCAM mRNA and protein level detected in NSCLC cells after re‐expression or knockdown of miR‐148b. (d,e) Relative luciferase activity in NSCLC cells after transfection with miR‐148b mimic or inhibitor. (f) RT‐PCR analysis of ALCAM level in NSCLC tissues. (g) RT‐PCR analysis of ALCAM level in NSCLC cell lines. (h) Repression analysis of correlation of miR‐148b with ALCAM. (b, c, e) () control mimic, () miR‐148b mimic, () control inhibitor and () miR‐148b inhibitor.