| Component | Amount per reaction (μl) | Final concentration |
|---|---|---|
| 10 mM 4-thiouracil solution | 1 | |
| DMSO | 5 | 50% (vol/vol) |
| 100 mM IAA (or EtOH for negative control) |
1 | 10 mM |
| 500 mM sodium phosphate, pH 8 | 1 | 50 mM |
| Nuclease-free water | 2 | |
| Total | 10 | |
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