Component | Amount per reaction (μl) | Final concentration |
---|---|---|
Nuclease-free water |
3 | |
2X PowerUp SYBR Green master Mix | 5 | 1X |
10 μM forward primer | 0.5 | 1 μM |
10 μM reverse primer | 0.5 | 1 μM |
| ||
Total | 9 |
Component | Amount per reaction (μl) | Final concentration |
---|---|---|
Nuclease-free water |
3 | |
2X PowerUp SYBR Green master Mix | 5 | 1X |
10 μM forward primer | 0.5 | 1 μM |
10 μM reverse primer | 0.5 | 1 μM |
| ||
Total | 9 |