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. Author manuscript; available in PMC: 2020 Feb 3.
Published in final edited form as: Nat Protoc. 2019 Jun 26;14(8):2261–2278. doi: 10.1038/s41596-019-0179-x
Component Amount per reaction (μl) Final concentration

Nuclease-free water
3
2X PowerUp SYBR Green master Mix 5 1X
10 μM forward primer 0.5 1 μM
10 μM reverse primer 0.5 1 μM

Total 9