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. Author manuscript; available in PMC: 2020 Feb 3.
Published in final edited form as: Nat Protoc. 2019 Jun 3;14(7):1991–2014. doi: 10.1038/s41596-019-0169-z

Table 2. Mutagenesis systems and enzymes.

Name Enzymes 1 (E1-1; E1-2) Enzyme 2 (E2) Mapping strategy
Lenti-EGT NlaIII; TaqI PacI 5’
Retro-EGT NlaIII; MseI SbfI 5’
Tol2-EGT NlaIII; TaqI PacI 3’
Tol2-polyA-EGT NlaIII; TaqI PacI 3’

EGT, enhanced gene trap