Table 2. Mutagenesis systems and enzymes.
Name | Enzymes 1 (E1-1; E1-2) | Enzyme 2 (E2) | Mapping strategy |
---|---|---|---|
Lenti-EGT | NlaIII; TaqI | PacI | 5’ |
Retro-EGT | NlaIII; MseI | SbfI | 5’ |
Tol2-EGT | NlaIII; TaqI | PacI | 3’ |
Tol2-polyA-EGT | NlaIII; TaqI | PacI | 3’ |
EGT, enhanced gene trap